Evaluation of a Loop-Mediated Isothermal Amplification assay to detect carbapenemases from Acinetobacter spp. directly from bronchoalveolar lavage fluid

Moreno-Morales, J.; Vergara, A.; Kostyanev, T.; Rodriguez-Bano, J.; Goossens, H.; Vila, J.

doi:10.1101/2020.05.11.089904

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bioRxiv · 10.1101/2020.05.11.089904

Evaluation of a Loop-Mediated Isothermal Amplification assay to detect carbapenemases from Acinetobacter spp. directly from bronchoalveolar lavage fluid

Moreno-Morales, J.·Vergara, A.·Kostyanev, T.·Rodriguez-Bano, J.·Goossens, H.·Vila, J.

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Abstract

Carbapenem-resistant Acinetobacter spp. mainly Acinetobacter baumannii are frequently causing nosocomial infections with high mortality. In this study, the efficacy of the Eazyplex(R) SuperBug Complete A system (Amplex Diagnostics GmbH, Gars-Bahnhof, Germany), based on loop-mediated isothermal amplification (LAMP), to detect the presence of carbapenemases in Acinetobacter spp. directly from bronchoalveolar lavage samples was assessed, detecting all tested carbapenemases in less than 30 minutes with a sensitivity of 103 CFU/ml.

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Moreno-Morales, J., Vergara, A., Kostyanev, T., Rodriguez-Bano, J., Goossens, H., Vila, J.. 2020-05-15. Evaluation of a Loop-Mediated Isothermal Amplification assay to detect carbapenemases from Acinetobacter spp. directly from bronchoalveolar lavage fluid. https://doi.org/10.1101/2020.05.11.089904

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