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bioRxiv · 10.1101/2020.05.11.089136

Nicotinamide mononucleotide redox cofactor system enables aldehyde accumulation in Escherichia coli

Richardson, K. N.·Black, W. B.·Li, H.
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Abstract

It is challenging to biosynthesize industrially important aldehydes, which are readily consumed by the numerous alcohol dehydrogenases (ADHs) in cells. In this work, we demonstrate that a nicotinamide mononucleotide (NMN+)-dependent redox cofactor cycling system enables aldehyde accumulation in Escherichia coli crude lysates and whole cells. By specifically delivering reducing power to a recombinant enoate reductase, but not to endogenous ADHs, we convert citral to citronellal with minimal byproduct formation (98% and 83% product purity in crude lysate- and whole cell-based biotransformation, respectively). We envision the systems universal application to lower the noise in biomanufacturing by silencing the hosts metabolic background.

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Richardson, K. N., Black, W. B., Li, H.. 2020-05-11. Nicotinamide mononucleotide redox cofactor system enables aldehyde accumulation in Escherichia coli. https://doi.org/10.1101/2020.05.11.089136

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