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bioRxiv · 10.1101/2020.05.07.082214

High-Dimensional Immunophenotyping of Murine T-cell and B-cell Subsets

Mincham, K. T.·Young, J. D.·Strickland, D. H.
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Abstract

Purpose and appropriate sample typesThis 19-parameter, 18-colour flow cytometry panel was designed and optimised to enable the comprehensive and simultaneous immunophenotyping of distinct T-cell and B-cell subsets within murine lymphoid tissues (Table 1). Cellular populations identified by employing this OMIP include 4 major subsets of B-cells (memory, activated, plasma cells and plasmablasts) and 7 major subsets of CD4+ T-cells (naive, central memory, effector memory, helper, regulatory, follicular helper and follicular regulatory). Staining was performed on freshly isolated splenocytes from 21-day-old neonatal BALB/c mice, however due to the omission of mouse strain-specific markers, this OMIP can be implemented across a range of murine models where in-depth immunophenotyping of the diverse repertoire of T-cell and B-cell populations localised within lymphoid tissues is required. O_TBL View this table: org.highwire.dtl.DTLVardef@1b488fdorg.highwire.dtl.DTLVardef@18dd356org.highwire.dtl.DTLVardef@2dce91org.highwire.dtl.DTLVardef@154cc27org.highwire.dtl.DTLVardef@27237c_HPS_FORMAT_FIGEXP M_TBL O_FLOATNOTable 1.C_FLOATNO O_TABLECAPTIONSummary Table C_TABLECAPTION C_TBL

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Mincham, K. T., Young, J. D., Strickland, D. H.. 2020-05-07. High-Dimensional Immunophenotyping of Murine T-cell and B-cell Subsets. https://doi.org/10.1101/2020.05.07.082214

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