Identifying the effect of vancomycin on HA-MRSA strains using bacteriological and physiological media

Rajput, A.; Poudel, S.; Tsunemoto, H.; Meehan, M.; Szubin, R.; Olson, C.; Seif, Y.; Lamsa, A.; Dillon, N.; Vrbanac, A.; Sugie, J.; Dahesh, S.; Monk, J.; Dorrestein, P.; Pogliano, J.; Nizet, V.; Feist, A.; Palsson, B. O.

doi:10.1101/2020.05.06.079640

bioRxiv · 10.1101/2020.05.06.079640

Identifying the effect of vancomycin on HA-MRSA strains using bacteriological and physiological media

Rajput, A.·Poudel, S.·Tsunemoto, H.·Meehan, M.·Szubin, R.·Olson, C.·Seif, Y.·Lamsa, A.·Dillon, N.·Vrbanac, A.·Sugie, J.·Dahesh, S.·Monk, J.·Dorrestein, P.·Pogliano, J.·Nizet, V.·Feist, A.·Palsson, B. O.

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Abstract

Healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern due to their evolving antibiotic resistant. They are resistant to a broad class of antibiotics like macrolides, aminoglycosides, fluoroquinolones, and many more. The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (CA-MHB) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behaviour of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments. The study reveals the vancomycin resistance behavior of USA100 strains in dual media conditions using wide-ranging experiments.

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Rajput, A., Poudel, S., Tsunemoto, H., Meehan, M., Szubin, R., Olson, C., Seif, Y., Lamsa, A., Dillon, N., Vrbanac, A., Sugie, J., Dahesh, S., Monk, J., Dorrestein, P., Pogliano, J., Nizet, V., Feist, A., Palsson, B. O.. 2020-05-07. Identifying the effect of vancomycin on HA-MRSA strains using bacteriological and physiological media. https://doi.org/10.1101/2020.05.06.079640

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