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bioRxiv · 10.1101/2020.04.30.070573

Optimization of Human Papillomavirus-based Pseudovirus Techniques for Efficient Gene Transfer

Gilson, T. D.·Gibson, R.·Androphy, E. J.
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Abstract

Human papillomavirus (HPV) L1 and L2 capsid proteins self-assemble into virions capable of efficiently packaging either its 8 kilobase genome or non-viral DNA. The ability of HPV capsids to package non-viral DNA makes these a useful tool for delivering plasmids to study proteins of interest in a variety of cell types. We describe optimization of current methods and present new protocols for using HPV capsids to deliver non-viral DNA thereby providing an alternative to DNA transfection. Using keratinocyte generated extracellular matrices can enhance infection efficiency in keratinocytes, hepatocytes and neuronal cells. Furthermore, we describe a suspension-based efficient technique for infecting different cell types.

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Gilson, T. D., Gibson, R., Androphy, E. J.. 2020-05-01. Optimization of Human Papillomavirus-based Pseudovirus Techniques for Efficient Gene Transfer. https://doi.org/10.1101/2020.04.30.070573

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