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bioRxiv · 10.1101/2020.04.29.069534

Conserved +1 translational frameshifting in the S. cerevisiae gene encoding YPL034W

Ivanov, I. P.·Gaikwad, S.·Hinnebusch, A. G.·Dever, T. E.
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Abstract

Living cells have developed exquisite mechanisms to ensure accurate translation of mRNA. Many of them are dedicated to preventing the change in reading frame during translation elongation. A minority of chromosomally encoded genes have evolved sequences that subvert standard decoding to program +1 translational frameshifting, either constitutively or in response to external stimuli. In the yeast Saccharomyces cerevisiae, three chromosomal genes are known to employ programmed +1 translational frameshifting for expression of full-length functional products. Here we identify a fourth yeast gene, YFS1, encompassing the existing predicted open reading frame YPL034W, with conserved programmed +1 frameshifting. Like the previously known examples, it appears to exploit peculiarities in tRNA abundance in S. cerevisiae.

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Ivanov, I. P., Gaikwad, S., Hinnebusch, A. G., Dever, T. E.. 2020-05-01. Conserved +1 translational frameshifting in the S. cerevisiae gene encoding YPL034W. https://doi.org/10.1101/2020.04.29.069534

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