Titration of in-cellula affinities of protein-protein interactions

Cluet, D.; Vergier, B.; Levy, N.-P.; Dehau, L.; Thurman, A.; Amri, I.; Spichty, M.

doi:10.1101/2020.04.27.063552

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bioRxiv · 10.1101/2020.04.27.063552

Titration of in-cellula affinities of protein-protein interactions

Cluet, D.·Vergier, B.·Levy, N.-P.·Dehau, L.·Thurman, A.·Amri, I.·Spichty, M.

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Abstract

A genetic assay permits simultaneous quantification of two interacting proteins and their bound fraction at the single-cell level using flow cytometry. In-cellula affinities of protein-protein interactions can be extracted from the acquired data through a titration-like analysis. The applicability of this approach is demonstrated on a diverse set of interactions with proteins from different families and organisms and with in-vitro dissociation constants ranging from picomolar to micromolar.

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Cluet, D., Vergier, B., Levy, N.-P., Dehau, L., Thurman, A., Amri, I., Spichty, M.. 2020-04-29. Titration of in-cellula affinities of protein-protein interactions. https://doi.org/10.1101/2020.04.27.063552

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