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bioRxiv · 10.1101/2020.04.24.059410

Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy

Clowsley, A. H.·Kaufhold, W. T.·Lutz, T.·Meletiou, A.·Di Michele, L.·Soeller, C.
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Abstract

DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA imagers to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.

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Clowsley, A. H., Kaufhold, W. T., Lutz, T., Meletiou, A., Di Michele, L., Soeller, C.. 2020-04-25. Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy. https://doi.org/10.1101/2020.04.24.059410

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