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bioRxiv · 10.1101/2020.04.22.055897

Rapid, sensitive, full genome sequencing of Severe Acute Respiratory Syndrome Virus Coronavirus 2 (SARS-CoV-2)

Paden, C. R.·Tao, Y.·Queen, K.·Zhang, J.·Li, Y.·Uehara, A.·Tong, S.
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Abstract

SARS-CoV-2 recently emerged, resulting a global pandemic. Rapid genomic information is critical to understanding transmission and pathogenesis. Here, we describe validated protocols for generating high-quality full-length genomes from primary samples. The first employs multiplex RT-PCR followed by MinION or MiSeq sequencing. The second uses singleplex, nested RT-PCR and Sanger sequencing.

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Paden, C. R., Tao, Y., Queen, K., Zhang, J., Li, Y., Uehara, A., Tong, S.. 2020-04-24. Rapid, sensitive, full genome sequencing of Severe Acute Respiratory Syndrome Virus Coronavirus 2 (SARS-CoV-2). https://doi.org/10.1101/2020.04.22.055897

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