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bioRxiv · 10.1101/2020.04.10.035295

A high-throughput screening platform for pigment regulating agents using pluripotent stem cell derived melanocytes.

Parat, V.·Onteniente, B.·Maruotti, J.
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Abstract

In this study, we describe a simple and straight-forward assay using induced pluripotent stem cell derived melanocytes and high-throughput flow cytometry, to screen and identify pigment regulating agents. The assays is based on the correlation between forward light-scatter characteristics and melanin content, with pigmented cells displaying high light absorption/low forward light-scatter, while the opposite is true for lowly pigmented melanocytes, as a result of genetic background or chemical treatments. Orthogonal validation is then performed by regular melanin quantification. Such approach was validated using a set of 80 small molecules, and yielded a confirmed hit. The assay described in this study may prove a useful tool to identify modulators of melanogenesis in human melanocytes.

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Parat, V., Onteniente, B., Maruotti, J.. 2020-04-10. A high-throughput screening platform for pigment regulating agents using pluripotent stem cell derived melanocytes.. https://doi.org/10.1101/2020.04.10.035295

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