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bioRxiv · 10.1101/2020.04.10.023358

SARS-CoV-2 detection with CRISPR diagnostics

Guo, L.·Sun, X.·Wang, X.·Liang, C.·Jiang, H.·Gao, Q.·Dai, M.·Qu, B.·Fang, S.·Mao, Y.·Chen, Y.·Feng, G.·Gu, Q.·Wang, L.·Wang, R. R.·Zhou, Q.·Li, W.
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Abstract

The novel coronavirus (CoV) disease termed COVID-19 (Coronavirus Disease-19) caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) is causing a massive pandemic worldwide, threatening public health systems across the globe. During this ongoing COVID-19 outbreak, nucleic acid detection has played an important role in early diagnosis. Here we report a SARS-CoV-2 detection protocol using a CRISPR-based CRISPR diagnostic platform - CDetection (Cas12b-mediated DNA detection). By combining sample treatment protocols and nucleic acid amplification methods with CDetection, we have established an integrated viral nucleic acid detection platform - CASdetec (CRISPR-assisted detection). The detection limit of CASdetec for SARS-CoV-2 pseudovirus is 1 x 104 copies/mL, with no cross reactivity observed. Our assay design and optimization process can provide guidance for future CRISPR-based nucleic acid detection assay development and optimization.

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Guo, L., Sun, X., Wang, X., Liang, C., Jiang, H., Gao, Q., Dai, M., Qu, B., Fang, S., Mao, Y., Chen, Y., Feng, G., Gu, Q., Wang, L., Wang, R. R., Zhou, Q., Li, W.. 2020-04-11. SARS-CoV-2 detection with CRISPR diagnostics. https://doi.org/10.1101/2020.04.10.023358

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