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bioRxiv · 10.1101/2020.04.08.031427

A chemical strategy to control protein networks in vivo

Ziegler, M. J.·Yserentant, K.·Middel, V.·Dunsing, V.·Gralak, A. J.·Pakari, K.·Bargstedt, J.·Kern, C.·Chiantia, S.·Straehle, U.·Herten, D.-P.·Wombacher, R.
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Abstract

Direct control of protein interaction by chemically induced protein proximity (CIPP) holds great potential for cell- and synthetic biology as well as therapeutic applications. However, toxicity, low cell-permeability and lack of orthogonality currently limit the use of available chemical inducers of proximity (CIP). We present Mandi, a novel CIP and demonstrate its applicability in cell culture systems as well as living organisms for protein translocation, protein network shuttling and manipulation of endogenous proteins.

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Ziegler, M. J., Yserentant, K., Middel, V., Dunsing, V., Gralak, A. J., Pakari, K., Bargstedt, J., Kern, C., Chiantia, S., Straehle, U., Herten, D.-P., Wombacher, R.. 2020-04-09. A chemical strategy to control protein networks in vivo. https://doi.org/10.1101/2020.04.08.031427

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