A novel antiviral formulation inhibits a range of enveloped viruses.

Fletcher, N. F.; Meredith, L. W.; Tidswell, E. L.; Bryden, S. R.; Goncalves-Carneiro, D.; Chaudhry, Y.; Shannon-Lowe, C.; Folan, M. A.; Lefteri, D. A.; Pingen, M.; Bailey, D.; McKimmie, C. S.; Baird, A. W.

doi:10.1101/2020.03.29.009464

bioRxiv · 10.1101/2020.03.29.009464

A novel antiviral formulation inhibits a range of enveloped viruses.

Fletcher, N. F.·Meredith, L. W.·Tidswell, E. L.·Bryden, S. R.·Goncalves-Carneiro, D.·Chaudhry, Y.·Shannon-Lowe, C.·Folan, M. A.·Lefteri, D. A.·Pingen, M.·Bailey, D.·McKimmie, C. S.·Baird, A. W.

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Abstract

Some free fatty acids derived from milk and vegetable oils are known to have potent antiviral and antibacterial properties. However, therapeutic applications of short to medium chain fatty acids are limited by physical characteristics such as immiscibility in aqueous solutions. We evaluated a novel proprietary formulation based on an emulsion of short chain caprylic acid, ViroSAL, for its ability to inhibit a range of viral infections in vitro and in vivo. In vitro, ViroSAL inhibited the enveloped viruses Epstein-Barr, measles, herpes simplex, Zika and orf parapoxvirus, together with Ebola, Lassa, vesicular stomatitis and SARS-CoV-1 pseudoviruses, in a concentration- and time-dependent manner. Evaluation of the components of ViroSAL revealed that caprylic acid was the main antiviral component; however, the ViroSAL formulation significantly inhibited viral entry compared with caprylic acid alone. In vivo, ViroSAL significantly inhibited Zika and Semliki Forest Virus replication in mice following the inoculation of these viruses into mosquito bite sites. In agreement with studies investigating other free fatty acids, ViroSAL had no effect on norovirus, a non-enveloped virus, indicating that its mechanism of action may be via surfactant disruption of the viral envelope. We have identified a novel antiviral formulation that is of great interest for prevention and/or treatment of a broad range of enveloped viruses.

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Fletcher, N. F., Meredith, L. W., Tidswell, E. L., Bryden, S. R., Goncalves-Carneiro, D., Chaudhry, Y., Shannon-Lowe, C., Folan, M. A., Lefteri, D. A., Pingen, M., Bailey, D., McKimmie, C. S., Baird, A. W.. 2020-03-30. A novel antiviral formulation inhibits a range of enveloped viruses.. https://doi.org/10.1101/2020.03.29.009464

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