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bioRxiv · 10.1101/2020.03.24.006619

Sub-3 A resolution structure of apoferritin using a multi-purpose TEM with a side-entry cryo-holder

Kayama, Y.·Burton-Smith, R. N.·Song, C.·Terahara, N.·Kato, T.·Murata, K.
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Abstract

The structural analysis of protein complexes by cryo-electron microscopy (cryo-EM) single particle analysis (SPA) has had great impact as a biophysical method in recent years. Many results of cryo-EM SPA are based on state-of-the-art cryo-electron microscopes customized for SPA. These are currently only available in limited locations around the world, where securing machine time is highly competitive. One potential solution for this time-competitive situation is to reuse existing multi-purpose equipment. Here, we used a multi-purpose TEM with a side entry cryo-holder at our facility to evaluate the potential of high-resolution SPA. We report a 3 [A] resolution map of apoferritin with local resolution extending to 2.6 [A]. The map clearly showed two positions of an aromatic side chain. We also verified the optimal imaging conditions depending on different electron microscope and camera combinations. This study demonstrates the possibilities of more widely available and established electron microscopes, and their applications for cryo-EM SPA.

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Kayama, Y., Burton-Smith, R. N., Song, C., Terahara, N., Kato, T., Murata, K.. 2020-03-25. Sub-3 A resolution structure of apoferritin using a multi-purpose TEM with a side-entry cryo-holder. https://doi.org/10.1101/2020.03.24.006619

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