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bioRxiv · 10.1101/2020.03.19.998039

Click-ExM enables expansion microscopy for all biomolecules

Sun, D.-e.·Fan, X.·Zhang, H.·Huang, Z.·Tang, Q.·Li, W.·Bai, J.·Lei, X.·Chen, X.
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Abstract

Expansion microscopy (ExM) allows super-resolution imaging on conventional fluorescence microscopes, but has been limited to proteins and nucleic acids. Here we develop click-ExM, which integrates click-labeling into ExM to enable a "one-stop-shop" method for nanoscale imaging of various types of biomolecules. Using 18 clickable labels for click-ExM imaging of DNA, RNA, proteins, lipids, glycans and small molecules, we demonstrate its universality, compatibility with signal-amplification techniques, and broad applications in cellular imaging.

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Sun, D.-e., Fan, X., Zhang, H., Huang, Z., Tang, Q., Li, W., Bai, J., Lei, X., Chen, X.. 2020-03-20. Click-ExM enables expansion microscopy for all biomolecules. https://doi.org/10.1101/2020.03.19.998039

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