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bioRxiv · 10.1101/2020.03.19.993618

In vitro effects on cellular shaping, contratility, cytoskeletal organization and mitochondrial activity in HL1 cells after different sounds stimulation. A qualitative pilot study and a theoretical physical model.

Dal Lin, C.·Radu, C. M.·Vitiello, G.·Romano, P.·Polcari, A.·Iliceto, S.·Simioni, P.·Tona, F.
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Abstract

Convincing evidence has documented that mechanical vibrations profoundly affect the behaviour of different cell types and even the functions of different organs. Pressure waves such as those of sound could affect cytoskeletal molecules with coherent changes in their spatial organization and are conveyed to cellular nucleus via mechanotransduction. HL1 cells were grown and exposed to different sounds. Subsequently, cells were stained for phalloidin, beta-actin, alpha-tubulin, alpha-actinin-1 and MitoTracker(R) mitochondrial probe. The cells were analyzed with time-lapse and immunofluorescence/confocal microscopy. In this paper, we describe that different sound stimuli seem to influence the growth or death of HL1 cells, resulting in a different mitochondrial localization and expression of cytoskeletal proteins. Since the cellular behaviour seems to correlate with the meaning of the sound used, we speculate that it can be "understood" by the cells by virtue of the different sound waves geometric properties that we have photographed and filmed. A theoretical physical model is proposed to explain our preliminary results.

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Dal Lin, C., Radu, C. M., Vitiello, G., Romano, P., Polcari, A., Iliceto, S., Simioni, P., Tona, F.. 2020-03-20. In vitro effects on cellular shaping, contratility, cytoskeletal organization and mitochondrial activity in HL1 cells after different sounds stimulation. A qualitative pilot study and a theoretical physical model.. https://doi.org/10.1101/2020.03.19.993618

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