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bioRxiv · 10.1101/2020.03.19.988519

The microRNA, miR-133b, functions to slow Duchenne muscular dystrophy pathogenesis

Abstract

Duchenne muscular dystrophy (DMD) is characterized by progressive degeneration of skeletal muscles. To date, there are no treatments available to slow or prevent the disease. Hence, it remains essential to identify molecular factors that promote muscle biogenesis since they could serve as therapeutic targets for treating DMD. While the muscle enriched microRNA, miR-133b, has been implicated in the biogenesis of muscle fibers, its role in DMD remains unknown. To assess the role of miR-133b in DMD-affected skeletal muscles, we genetically ablated miR-133b in the mdx mouse model of DMD. In the absence of miR-133b, the tibialis anterior muscle of juvenile and adult mdx mice is populated by small muscle fibers with centralized nuclei, exhibits increased fibrosis, and thickened interstitial space. Additional analysis revealed that loss of miR-133b exacerbates DMD-pathogenesis partly by altering the number of satellite cells and levels of protein-encoding genes, including previously identified miR-133b targets as well as genes involved in cell proliferation and fibrosis. Altogether, our data demonstrate that skeletal muscles utilize miR-133b to mitigate the deleterious effects of DMD.

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BibTeXRIS

Taetzsch, T., Shapiro, D., Eldosougi, R., Myers, T., Settlage, R., Valdez, G.. 2020-03-20. The microRNA, miR-133b, functions to slow Duchenne muscular dystrophy pathogenesis. https://doi.org/10.1101/2020.03.19.988519

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