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bioRxiv · 10.1101/2020.03.09.980334

CryoSIM: super resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultra-structural imaging

Phillips, M. A.·Harkiolaki, M.·Pinto, D. M. S.·Parton, R. M.·Palanca, A.·Garcia-Moreno, M.·Sedat, J.·Stuart, D. I.·Castello, A.·Booth, M. J.·Davis, I.·Dobbie, I. M.
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Abstract

Rapid cryo-preservation of biological specimens is the gold standard for visualising cellular structures in their true structural context. However, current commercial cryo-fluorescence microscopes are limited to low resolutions. To fill this gap, we have developed cryoSIM, a microscope for 3D super-resolution fluorescence cryo-imaging for correlation with cryo electron microscopy or cryo soft X-ray tomography. We provide the full instructions for replicating the instrument mostly from off-the-shelf components and accessible, user-friendly open source Python control software. Therefore, cryoSIM democratises the ability to detect molecules using super-resolution fluorescence imaging of cryo-preserved specimens for correlation with their cellular ultrastructure.

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Phillips, M. A., Harkiolaki, M., Pinto, D. M. S., Parton, R. M., Palanca, A., Garcia-Moreno, M., Sedat, J., Stuart, D. I., Castello, A., Booth, M. J., Davis, I., Dobbie, I. M.. 2020-03-11. CryoSIM: super resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultra-structural imaging. https://doi.org/10.1101/2020.03.09.980334

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