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bioRxiv · 10.1101/2020.03.05.977991

Isoform specificity in the mouse primary motor cortex

Booeshaghi, A. S.·Yao, Z.·Velthoven, C. v.·Smith, K.·Tasic, B.·Zeng, H.·Pachter, L.
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Abstract

Full-length SMART-Seq single-cell RNA-seq can be used to measure gene expression at isoform resolution, making possible the identification of isoform markers for cell types and for an isoform atlas. In a comprehensive analysis of 6,160 mouse primary motor cortex cells assayed with SMART-Seq, we find numerous examples of isoform specificity in cell types, including isoform shifts between cell types that are masked in gene-level analysis. These findings can be used to refine spatial gene expression information to isoform resolution. Our results highlight the utility of full-length single-cell RNA-seq when used in conjunction with other single-cell RNA-seq technologies.

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Booeshaghi, A. S., Yao, Z., Velthoven, C. v., Smith, K., Tasic, B., Zeng, H., Pachter, L.. 2020-03-05. Isoform specificity in the mouse primary motor cortex. https://doi.org/10.1101/2020.03.05.977991

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