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bioRxiv · 10.1101/2020.03.01.972398

Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection

Azlan, A.·Yunus, M. A.·Azzam, G.
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Abstract

The Asian tiger mosquito, Aedes albopictus (Ae. albopictus), is a highly invasive species that transmit several arboviruses including dengue (DENV), Zika (ZIKV), and chikungunya (CHIKV). Although several studies have identified microRNAs (miRNAs) in Ae. albopictus, it is crucial to extend and improve current annotations with the newly improved genome assembly, and the increase number of small RNA-sequencing data. We combined our high-depth sequence data and 26 public datasets to re-annotate Ae. albopictus miRNAs, and found a total of 110 novel mature miRNAs. We discovered that the expression of novel miRNAs was lower than known miRNAs. Furthermore, compared to known miRNAs, novel miRNAs are prone to be expressed in stage-specific manner. Upon DENV infection, a total of 59 novel miRNAs were differentially expressed, and target prediction analysis revealed that miRNA-target genes were involved in lipid metabolism and protein processing in endoplasmic reticulum. Taken together, miRNA annotation profile provided here is the most comprehensive to date, and we believed that this will facilitate future research in understanding virus-host interactions particularly on the role of miRNAs.

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Azlan, A., Yunus, M. A., Azzam, G.. 2020-03-03. Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection. https://doi.org/10.1101/2020.03.01.972398

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