Increasing proteome coverage using cysteine-specific DIA Mass spectrometry - Cys-DIA

Tahir, M.; Nawrocki, A.; Ditzel, H. J.; Larsen, M. R.

doi:10.1101/2020.02.27.966861

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bioRxiv · 10.1101/2020.02.27.966861

Increasing proteome coverage using cysteine-specific DIA Mass spectrometry - Cys-DIA

Tahir, M.·Nawrocki, A.·Ditzel, H. J.·Larsen, M. R.

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Abstract

We report a Data Independent Analysis (DIA) mass spectrometric method for quantitative proteomics based on cysteine-containing peptides derived by tryptic digestion from biological samples (termed Cys-DIA). This technology significantly increases the coverage of the identifiable and quantifiable proteome in DIA/SWATH analyses by >35% compared to the conventional DIA/SWATH methods. The strategy was applied here to illustrate the quantitative difference in the proteome between isogenic human cancer cell lines with different metastatic capabilities.

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Tahir, M., Nawrocki, A., Ditzel, H. J., Larsen, M. R.. 2020-02-27. Increasing proteome coverage using cysteine-specific DIA Mass spectrometry - Cys-DIA. https://doi.org/10.1101/2020.02.27.966861

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