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bioRxiv · 10.1101/2020.02.20.957860

Quantitative analyses reveal extracellular dynamics of Wnt ligands in Xenopus embryos

Mii, Y.·Nakazato, K.·Pack, C.-G.·Sako, Y.·Mochizuki, A.·Takada, S.·Taira, M.
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Abstract

The mechanism of intercellular transport of Wnt ligands is still a matter of debate. Here, to better understand this issue, we examined distribution and dynamics of Wnt8 in Xenopus embryos. While Venus-tagged Wnt8 was found on the surfaces of cells close to Wnt-producing cells, we also detected its dispersal over distances of 15 cell diameters. A combination of fluorescence correlation spectroscopy and quantitative imaging revealed that only a small proportion of Wnt8 ligands diffuses freely, whereas most Wnt8 molecules are bound to cell surfaces. Fluorescence decay after photoconversion showed that Wnt8 ligands bound on cell surfaces decreased exponentially, suggesting a dynamic exchange of bound forms of Wnt ligands. Mathematical modelling based on this exchange recapitulates a graded distribution of bound, but not free, Wnt ligands. Based on these results, we propose that Wnt distribution in tissues is controlled by a dynamic exchange of its abundant bound and rare free populations.

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Mii, Y., Nakazato, K., Pack, C.-G., Sako, Y., Mochizuki, A., Takada, S., Taira, M.. 2020-02-20. Quantitative analyses reveal extracellular dynamics of Wnt ligands in Xenopus embryos. https://doi.org/10.1101/2020.02.20.957860

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