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bioRxiv · 10.1101/2020.02.13.948455

A one-tube method for rapid and reliable plant genomic DNA isolation for PCR analysis

Hu, W.·Lagarias, J. C.
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Abstract

BackgroundConsistent isolation of high quality plant genomic DNA is a prerequisite for successful PCR analysis. Time consumption, ease of operation and procedure cost are important secondary considerations for selecting an effective DNA extraction method. The simple, reliable and rapid DNA extraction method developed by Edwards and colleagues in 1991 [1] has proven to be the gold standard. ResultsThrough modification of the Edwards method of extraction, we have developed a one-tube protocol that greatly improves the efficiency of plant DNA extraction and reduces the potential for sample contamination while simultaneously yielding high quality DNA suitable for PCR analysis. We further show that DNA extracts prepared with this method are stable at room temperature for at least three months. ConclusionThe one-tube extraction method yields high quality plant DNA with improved efficiency while greatly minimizing the potential for cross contamination. This low-cost and environment-friendly method is widely applicable for plant molecular biology research.

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Hu, W., Lagarias, J. C.. 2020-02-14. A one-tube method for rapid and reliable plant genomic DNA isolation for PCR analysis. https://doi.org/10.1101/2020.02.13.948455

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