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bioRxiv · 10.1101/2020.02.12.944066

Structure and function of yeast Lso2 and human CCDC124 bound to hibernating ribosomes.

Wells, J. N.·Buschauer, R.·Mackens-Kiani, T.·Best, K.·Kratzat, H.·Berninghausen, O.·Becker, T.·Gilbert, W. V.·Cheng, J.·Beckmann, R.
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Abstract

Cells adjust to nutrient deprivation by reversible translational shut down. This is accompanied by maintaining inactive ribosomes in a hibernation state, where they are bound by proteins with inhibitory and protective functions. In eukaryotes, such a function was attributed to Stm1 (SERBP1 in mammals), and recently Lso2 (CCDC124 in mammals) was found to be involved in translational recovery after starvation from stationary phase. Here, we present cryo-electron microscopy (cryo-EM) structures of translationally inactive yeast and human ribosomes. We found Lso2/CCDC124 accumulating on idle ribosomes in the non-unrotated state, in contrast to Stm1/SERBP1-bound ribosomes, which display a rotated state. Lso2/CCDC124 bridges the decoding sites of the small with the GTPase-activating center of the large subunit. This position allows accommodation of the Dom34-dependent ribosome recycling system, which splits Lso2-containing but not Stm1-containing ribosomes. We propose a model in which Lso2 facilitates rapid translation reactivation by stabilizing the recycling-competent state of inactive ribosomes.

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Wells, J. N., Buschauer, R., Mackens-Kiani, T., Best, K., Kratzat, H., Berninghausen, O., Becker, T., Gilbert, W. V., Cheng, J., Beckmann, R.. 2020-02-12. Structure and function of yeast Lso2 and human CCDC124 bound to hibernating ribosomes.. https://doi.org/10.1101/2020.02.12.944066

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