Acoustic Ejection Mass Spectrometry for High-Throughput Analysis

Zhang, H.; Liu, C.; Hua, W.; Ghislain, L. P.; Liu, J.; Aschenbrenner, L.; Noell, S.; Dirico, K.; Lanyon, L. F.; Steppan, C. M.; Arnold, D. W.; Covey, T. R.; Datwani, S. S.; Troutman, M. D.

doi:10.1101/2020.01.28.923938

bioRxiv · 10.1101/2020.01.28.923938

Acoustic Ejection Mass Spectrometry for High-Throughput Analysis

Zhang, H.·Liu, C.·Hua, W.·Ghislain, L. P.·Liu, J.·Aschenbrenner, L.·Noell, S.·Dirico, K.·Lanyon, L. F.·Steppan, C. M.·Arnold, D. W.·Covey, T. R.·Datwani, S. S.·Troutman, M. D.

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Abstract

We describe a mass spectrometry (MS) analytical platform resulting from the novel integration of acoustic droplet ejection (ADE) technology, an open-port interface (OPI), and electrospray ionization (ESI) MS that creates a transformative system enabling high-speed sampling and label-free analysis. The ADE technology delivers nanoliter droplets in a touchless manner with high speed, precision and accuracy; subsequent sample dilution within the OPI, in concert with the capabilities of modern ESI-MS, eliminates the laborious sample preparation and method development required in current approaches. This platform is applied to a variety of experiments, including high-throughput (HT) pharmacology screening, label-free in situ enzyme kinetics, in vitro and in vivo adsorption, distribution, metabolism, elimination, pharmacokinetic (PK) and biomarker analysis, and HT parallel medicinal chemistry. One Sentence SummaryADE-OPI-MS is a transformational analytical platform that increases mass spectrometry utility via sub-second speed and non-contact sampling.

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Zhang, H., Liu, C., Hua, W., Ghislain, L. P., Liu, J., Aschenbrenner, L., Noell, S., Dirico, K., Lanyon, L. F., Steppan, C. M., Arnold, D. W., Covey, T. R., Datwani, S. S., Troutman, M. D.. 2020-01-29. Acoustic Ejection Mass Spectrometry for High-Throughput Analysis. https://doi.org/10.1101/2020.01.28.923938

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