Responsive fluorophore aggregation provides spectral contrast for fluorescence lifetime imaging

Schleyer, K. A.; Datko, B. D.; Burnside, B.; Cui, C.; Ma, X.; Grey, J. K.; Cui, L.

doi:10.1101/2020.01.28.923672

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bioRxiv · 10.1101/2020.01.28.923672

Responsive fluorophore aggregation provides spectral contrast for fluorescence lifetime imaging

Schleyer, K. A.·Datko, B. D.·Burnside, B.·Cui, C.·Ma, X.·Grey, J. K.·Cui, L.

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Abstract

Fluorophores experience altered emission lifetimes when incorporated into and liberated from macromolecules or molecular aggregates; this trend suggests the potential for a fluorescent, responsive probe capable of undergoing self-assembly and aggregation and consequently altering the lifetime of its fluorescent moiety to provide contrast between the active and inactive probes. We developed a cyanobenzothioazole-fluorescein conjugate (1), and spectroscopically examined the lifetime changes caused by its reduction-induced aggregation in vitro. A decrease in lifetime was observed for compound 1 in a buffered system activated using the biological reducing agent glutathione, suggesting a possible approach for designing responsive self-aggregating lifetime imaging probes.

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Schleyer, K. A., Datko, B. D., Burnside, B., Cui, C., Ma, X., Grey, J. K., Cui, L.. 2020-01-29. Responsive fluorophore aggregation provides spectral contrast for fluorescence lifetime imaging. https://doi.org/10.1101/2020.01.28.923672

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