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bioRxiv · 10.1101/2020.01.24.918623

The Wg and Dpp morphogens regulate gene expression by modulating the frequency of transcriptional bursts

Bakker, R.·Mani, M.·Carthew, R. W.
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Abstract

Morphogen signaling contributes to the proper spatiotemporal expression of genes during development. One method of regulation of signaling-responsive genes is at the level of transcription. Single-cell quantitative studies of transcription have revealed that transcription occurs intermittently, in bursts. Although the effects of many gene regulatory mechanisms on transcriptional bursting have been studied, it remains unclear how morphogen gradients affect this dynamic property of downstream genes. Here we have adapted smFISH for use in the Drosophila wing imaginal disc in order to measure nascent and mature mRNA of genes downstream of the Wg and Dpp morphogen gradients. We compared our experimental results with predictions from stochastic models of transcription. Our results indicate that the transcription levels of these genes appear to share a common method of control via burst frequency modulation. Our data helps further elucidate the link between developmental gene regulatory mechanisms and transcriptional bursting.

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Bakker, R., Mani, M., Carthew, R. W.. 2020-01-25. The Wg and Dpp morphogens regulate gene expression by modulating the frequency of transcriptional bursts. https://doi.org/10.1101/2020.01.24.918623

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