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bioRxiv · 10.1101/2020.01.24.917807

PAKC: A novel Panel of HLA class I Antigen presentation machinery Knockout Cells from the same genetic origin

Abstract

With the emergence of immunotherapies, the understanding of functional HLA class I antigen presentation to T cells is more relevant than ever. Current knowledge on antigen presentation is based on decades of research in a wide variety of cell types with varying antigen presentation machinery (APM) expression patterns, proteomes and HLA haplotypes. This diversity complicates the establishment of individual APM contributions to antigen generation, selection and presentation. Therefore, we generated a novel Panel of APM Knockout Cell lines (PAKC) from the same genetic origin. After CRISPR/Cas9 genome-editing of ten individual APM components in a human cell line, we derived clonal cell lines and confirmed their knockout status and phenotype. We then show how PAKC will accelerate research on the functional interplay between APM components and their role in antigen generation and presentation. This will lead to improved understanding of peptide-specific T cell responses in infection, cancer and autoimmunity. Key pointsO_LIWe generated a panel of cell lines to study HLA class I antigen presentation C_LIO_LIWe show how this will spark research in infection, tumor biology and autoimmunity C_LI

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BibTeXRIS

de Waard, A. A., Verkerk, T., Jongsma, M. L. M., Hoefakker, K., Sethumadhavan, S., Gerke, C., Bliss, S., Janssen, G. M. C., de Ru, A. H., Claas, F. H. J., Mulder, A., Tampe, R., van Veelen, P. A., Halenius, A., Spaapen, R. M.. 2020-01-25. PAKC: A novel Panel of HLA class I Antigen presentation machinery Knockout Cells from the same genetic origin. https://doi.org/10.1101/2020.01.24.917807

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