MK2-deficient mice are bradycardic and display delayed hypertrophic remodelling in response to a chronic increase in afterload

Ruiz, M.; Khairallah, M.; Dingar, D.; Vaniotis, G.; Khairallah, R. J.; Lauzier, B.; Thibault, S.; Trepanier, J.; Shi, Y.; Douillette, A.; Hussein, B.; Nawaito, S. A.; Sahadevan, P.; Nguyen, A.; Gillis, M.-A.; Sirois, M. G.; Gaestel, M.; Stanley, W. C.; Fiset, C.; Tardif, J.-C.; Allen, B. G.

doi:10.1101/2020.01.23.916049

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bioRxiv · 10.1101/2020.01.23.916049

MK2-deficient mice are bradycardic and display delayed hypertrophic remodelling in response to a chronic increase in afterload

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Abstract

MAP kinase-activated protein kinase-2 (MK2) is protein serine/threonine kinase activated by p38/{beta}. Herein we examined the cardiac phenotype of pan MK2-null (MK2-/-) mice. Survival curves for male MK2+/+ and MK2-/- mice did not differ (Mantel-Cox test, P = 0.580). At 12-weeks of age, MK2-/- mice exhibited normal systolic function along with signs of possible early diastolic dysfunction; however, ageing was not associated with an abnormal reduction in diastolic function. Both R-R interval and P-R segment durations were prolonged in MK2-deficient mice. However, heart rates normalized when isolated hearts were perfused ex vivo in working mode. Ca2+ transients evoked by field stimulation or caffeine were similar in ventricular myocytes from MK2+/+ and MK2-/- mice. MK2-/- mice had lower body temperature and an age-dependent reduction in body weight. mRNA levels of key metabolic genes, including Ppargc1a, Acadm, Lipe, and Ucp3 were increased in hearts from MK2-/- mice. For equivalent respiration rates, mitochondria from MK2-/- hearts showed a significant decrease in Ca2+-sensitivity to mitochondrial permeability transition pore (mPTP) opening. Finally, the pressure overload-induced increase in heart weight/tibia length and decrease in systolic function were attenuated in MK2-/- mice two weeks, but not eight weeks, after constriction of the transverse aorta. Collectively, these results implicate MK2 in (i) autonomic regulation of heart rate, (ii) cardiac mitochondrial function, and (iii) the early stages of myocardial remodeling in response to chronic pressure overload. Key points summaryThe cardiac characterization of pan MK2-null mice showed: O_LIaltered autonomic regulation of heart rate C_LIO_LIincreased expression of key metabolic genes C_LIO_LIdecreased Ca2+-sensitivity for MPTP opening C_LIO_LIdelayed hypertrophic remodeling in response to increased afterload C_LI

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Ruiz, M., Khairallah, M., Dingar, D., Vaniotis, G., Khairallah, R. J., Lauzier, B., Thibault, S., Trepanier, J., Shi, Y., Douillette, A., Hussein, B., Nawaito, S. A., Sahadevan, P., Nguyen, A., Gillis, M.-A., Sirois, M. G., Gaestel, M., Stanley, W. C., Fiset, C., Tardif, J.-C., Allen, B. G.. 2020-01-24. MK2-deficient mice are bradycardic and display delayed hypertrophic remodelling in response to a chronic increase in afterload. https://doi.org/10.1101/2020.01.23.916049

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