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bioRxiv · 10.1101/2020.01.22.916346

Novel culture method to diagnose fungal infections with biopsy tissues

Zhao, J.·Zhang, Y.·Fan, Y.·Xiong, Z.·Xia, Y.·Zou, X.·Han, J.·Li, B. B.·Wang, C.·Liu, X.·Lu, B.·Cao, B.
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Abstract

Biopsy tissue is a difficult sample to gain for the identification of potential pathogen in the clinical laboratory. At present, there are no effective culture methods for small-sized biopsy tissue. In this study, we propose a novel tissue biopsy culture method based on Myco/F lytic liquid culture system, namely, tissue was grinded before injected into culture bottles. More types and numbers of fungi are cultivated using this culture system than sputum or BALF culture. A few of mycobacteria were also detected with ground tissues. The method may be a promising alternative to or supplement for the traditional plate culture in clinical practice.

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Zhao, J., Zhang, Y., Fan, Y., Xiong, Z., Xia, Y., Zou, X., Han, J., Li, B. B., Wang, C., Liu, X., Lu, B., Cao, B.. 2020-01-23. Novel culture method to diagnose fungal infections with biopsy tissues. https://doi.org/10.1101/2020.01.22.916346

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