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bioRxiv · 10.1101/2020.01.19.911404

Multi-faceted deregulation of gene expression and protein synthesis with age

Anisimova, A. S.·Meerson, M. B.·Gerashchenko, M.·Kulakovskiy, I. V.·Dmitriev, S. E.·Gladyshev, V. N.
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Abstract

Protein synthesis represents a major metabolic activity of the cell. However, how it is affected by aging and how this in turn impacts cell function remains largely unexplored. To address this question, herein we characterized age-related changes in both the transcriptome and translatome of mouse tissues over the entire lifespan. Expression of the majority of differentially expressed genes followed a U-shaped curve with the turning point around 3-months-old. We showed that transcriptome changes govern changes in the translatome and are associated with altered expression of genes involved in inflammation, extracellular matrix and lipid metabolism. We also identified genes that may serve as candidate biomarkers of aging. At the translational level, we uncovered sustained down-regulation of a set of 5 terminal oligopyrimidine (5TOP) transcripts encoding protein synthesis and ribosome biogenesis machinery and regulated by the mTOR pathway. For many of them, ribosome occupancy dropped 3-fold or even more. Moreover, with age, ribosome coverage gradually decreased in the vicinity of start codons and increased near stop codons, revealing complex age-related changes in the translation process. Taken together, our results reveal systematic and multi-dimensional deregulation in protein synthesis, showing how this major cellular process declines with age.

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Anisimova, A. S., Meerson, M. B., Gerashchenko, M., Kulakovskiy, I. V., Dmitriev, S. E., Gladyshev, V. N.. 2020-01-19. Multi-faceted deregulation of gene expression and protein synthesis with age. https://doi.org/10.1101/2020.01.19.911404

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