Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis

Yoneyama, K.; Akiyama, K.; Brewer, P.; Mori, N.; Kawano-Kawada, M.; Haruta, S.; Nishiwaki, H.; Yamauchi, S.; Xie, X.; Umehara, M.; Beveridge, C. A.; Nomura, T.

doi:10.1101/2020.01.17.910877

bioRxiv · 10.1101/2020.01.17.910877

Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis

Yoneyama, K.·Akiyama, K.·Brewer, P.·Mori, N.·Kawano-Kawada, M.·Haruta, S.·Nishiwaki, H.·Yamauchi, S.·Xie, X.·Umehara, M.·Beveridge, C. A.·Nomura, T.

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Abstract

Strigolactones (SLs) regulate important aspects of plant growth and stress responses. Many diverse types of SL occur in plants, but a complete picture of biosynthesis remains unclear. In Arabidopsis thaliana, we have demonstrated that MAX1, a cytochrome P450 monooxygenase, converts carlactone (CL) into carlactonoic acid (CLA), and that LBO, a 2-oxoglutarate-dependent dioxygenase, converts methyl carlactonoate (MeCLA) into a metabolite called [MeCLA+16] Da. In the present study, feeding experiments with deuterated MeCLAs revealed that [MeCLA+16] Da is hydroxymethyl carlactonoate (1-HO-MeCLA). Importantly, this LBO metabolite was detected in plants. Interestingly, other related compounds, methyl 4-hydroxycarlactonoate (4-HO-MeCLA) and methyl 16-hydroxycarlactonoate (16-HO-MeCLA) were also found to accumulate in lbo mutants. 3-HO-, 4-HO- and 16-HO-CL were detected in plants, but their expected corresponding metabolites, HO-CLAs, were absent in max1 mutants. These results suggest that HO-CL derivatives are predominant SLs in Arabidopsis, produced through MAX1 and LBO.

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Yoneyama, K., Akiyama, K., Brewer, P., Mori, N., Kawano-Kawada, M., Haruta, S., Nishiwaki, H., Yamauchi, S., Xie, X., Umehara, M., Beveridge, C. A., Nomura, T.. 2020-01-18. Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis. https://doi.org/10.1101/2020.01.17.910877

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