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bioRxiv · 10.1101/2020.01.16.909267

Loss of Caveolin-1 and caveolae leads to increased cardiac cell stiffness and functional decline of the adult zebrafish heart

de la Pompa, J. L.·Grivas, D.·Gonzalez-Rajal, A.·Guerrero Rodriguez, C.·Garcia, R.
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Abstract

Caveolin-1 is the main structural protein of caveolae, small membrane invaginations involved in signal transduction and mechanoprotection. Here, we generated cav1-KO zebrafish lacking Cav1 and caveolae, and investigated the impact of this loss on adult heart function and response to cryoinjury. We found that cardiac function was impaired in adult cav1-KO fish, which showed a significantly decreased ejection fraction and heart rate. Using atomic force microscopy, we detected an increase in the stiffness of epicardial cells and cortical myocardium lacking Cav1/caveolae. This loss of cardiac elasticity might explain the decreased cardiac contraction and function. Surprisingly, cav1-KO mutants were able to regenerate their heart after a cryoinjury but showed a transient decrease in cardiomyocyte proliferation.

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de la Pompa, J. L., Grivas, D., Gonzalez-Rajal, A., Guerrero Rodriguez, C., Garcia, R.. 2020-01-16. Loss of Caveolin-1 and caveolae leads to increased cardiac cell stiffness and functional decline of the adult zebrafish heart. https://doi.org/10.1101/2020.01.16.909267

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