The role of startle fluctuation and non-response startle reflex in tracking amygdala dynamics

Abstract

The startle reflex is considered a sensitive defensive reaction to potential threats that manifests as a unique eye blink-like pattern in the EMG. Eye blink EMG has a bell-shaped potential when startle probes are elicited, that strongly corresponds to amygdala activity. Considering how amygdala activity fluctuates over time in response to emotional and self-threatening stimuli, observing startle eyeblink size fluctuation over time could provide a cost-effective, convenient, and less resource intensive means for examining amygdala activity over time. Yet based on current standards in the literature, often startle evoked eye blink EMG values do not exhibit activity 3SDs from the mean eyeblink response, thus these trials are typically excluded from startle analyses. It stands to reason, however, that these trials may still index amygdala activity in a meaningful way. Through investigating the association between startle eyeblink amplitude, corresponding ERP amplitude, and underlying neural activity, the current study provides evidence that startle amplitudes exhibit a linear relationship with proxies for amygdala activity, e.g., N100 amplitudes and regions heavily interconnected with the amygdala. Specifically, the startle reflex correlates to large amount of brain regions in N100 time window in addition to the N100 amplitude. Thus, both valid and otherwise traditionally non-valid startle reflex responses appear to index amygdala activity and should be included accordingly. This approach could help salvage large amounts of meaningful data traditionally excluded from studies interested in amygdala responses to various stimuli over time.