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bioRxiv · 10.1101/2020.01.03.894501

Dissociated hippocampal neurons exhibit distinct Zn2+ dynamics in a stimulation method-dependent manner

Sanford, L.·Palmer, A. E.
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Abstract

Ionic Zn2+ has increasingly been recognized as an important neurotransmitter and signaling ion in glutamatergic neuron pathways. Intracellular Zn2+ transiently increases as a result of neuronal excitation, and this Zn2+ signal is essential for neuron plasticity, but the source and regulation of the signal is still unclear. In this study we rigorously quantified Zn2+, Ca2+ and pH dynamics in dissociated mouse hippocampal neurons stimulated with bath application of high KCl or glutamate. While both stimulation methods yielded Zn2+ signals, Ca2+ influx, and acidification, glutamate stimulation induced more sustained high intracellular Ca2+ and a larger increase in intracellular Zn2+. However, the stimulation-induced pH change was similar between conditions, indicating that a different cellular change is responsible for the stimulation-dependent difference in Zn2+ signal. This work provides the first robust quantification of Zn2+ dynamics in neurons using different methods of stimulation.

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Sanford, L., Palmer, A. E.. 2020-01-04. Dissociated hippocampal neurons exhibit distinct Zn2+ dynamics in a stimulation method-dependent manner. https://doi.org/10.1101/2020.01.03.894501

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