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bioRxiv · 10.1101/2019.12.22.872002

Spatiotemporal analysis of host cell modification during herpes simplex virus 1 replication

Scherer, K. M.·Manton, J. D.·Soh, T. K.·Mascheroni, L.·Connor, V.·Crump, C. M.·Kaminski, C. F.
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Abstract

Herpesviruses are large and complex viruses, and have a long evolutionary history with their host species. One poorly understood, but important, area in virus-host interaction is the extensive remodelling of intracellular organelles and morphology that occurs during active viral replication. We have constructed a recombinant reporter virus that allowed us to classify four distinct stages in the infection cycle of herpes simplex virus 1. Single-cell analysis of the impact of the viral load on replication dynamics demonstrates the utility of this times-tamping method for tracking the infection cycle. High-resolution microscopy analysis of cellular structures in live and fixed cells in concert with our dual-fluorescent reporter virus have enabled us to generate a detailed overview over the spatial and temporal organisation of the cytoskeleton and organelles during viral replication. This provides the first systems-level analysis of the morphological changes involved in viral replication.

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Scherer, K. M., Manton, J. D., Soh, T. K., Mascheroni, L., Connor, V., Crump, C. M., Kaminski, C. F.. 2019-12-23. Spatiotemporal analysis of host cell modification during herpes simplex virus 1 replication. https://doi.org/10.1101/2019.12.22.872002

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