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bioRxiv · 10.1101/2019.12.16.876888

Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species

Abstract

The accumulation of amyloid Tau aggregates is implicated in Alzheimers disease and other Tauopathies. Molecular chaperones are known for their function in maintaining protein homeostasis by preventing the formation or promoting the disaggregation of amorphous and amyloid protein aggregates. Here we show that an ATP-dependent human chaperone system disassembles Tau fibrils in vitro. This function is mediated by the core chaperone Hsc70, assisted by specific co-chaperones, in particular class B J-domain proteins and an Hsp110-type NEF. Recombinant fibrils assembled from all six Tau isoforms as well as Sarkosyl-resistant Tau aggregates extracted from cell culture were processed by the Hsp70 disaggregation machinery, demonstrating the ability of this machinery to recognize a broad range of Tau aggregates. Chaperone treatment released monomeric, and small oligomeric Tau species, which induced the aggregation of self-propagating Tau species in a Tau cell culture model. We infer from these results that the activity of the Hsp70 disaggregation machinery is a double-sided sword as it attempts to eliminate Tau amyloids but with the price of generating new seeds. The Hsp70 disaggregase therefore has a crucial function in the Tau propagation cycle, rendering it a potential drug target in Tauopathies.

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BibTeXRIS

Nachman, E., Wentink, A., Madiona, K., Bousset, L., Katsinelos, T., Kampinga, H., McEwan, W. A., Jahn, T. R., Melki, R., Mogk, A., Bukau, B., Nussbaum-Krammer, C.. 2019-12-16. Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species. https://doi.org/10.1101/2019.12.16.876888

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