LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays

Benedetto, A. E.; Bambade, T.; Au, C. S. S.; Tullet, J. M.; Dang, H.; Cetnar, K.; Cabreiro, F. G.; Gems, D.

doi:10.1101/195347

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bioRxiv · 10.1101/195347

LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays

Benedetto, A. E.·Bambade, T.·Au, C. S. S.·Tullet, J. M.·Dang, H.·Cetnar, K.·Cabreiro, F. G.·Gems, D.

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Abstract

Caenorhabditis elegans is an excellent model for high-throughput experimental approaches, but lacks a robust, versatile and automated means to pinpoint time of death. Here we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode survival, which we apply to stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity and stress-resistance, and reveal an autophagy-dependent increase in acute stress resistance in early adulthood.

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Benedetto, A. E., Bambade, T., Au, C. S. S., Tullet, J. M., Dang, H., Cetnar, K., Cabreiro, F. G., Gems, D.. 2017-09-28. LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays. https://doi.org/10.1101/195347

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