Rats and axolotls share a common molecular signature after spinal cord injury enriched in collagen-1

Didangelos, A.; Bartus, K.; Tica, J.; Roschitzki, B.; Bradbury, E.

doi:10.1101/184713

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bioRxiv · 10.1101/184713

Rats and axolotls share a common molecular signature after spinal cord injury enriched in collagen-1

Didangelos, A.·Bartus, K.·Tica, J.·Roschitzki, B.·Bradbury, E.

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Abstract

Spinal cord injury (SCI) in mammals leads to irreversible tissue damage and loss of function. In contrast, axolotls are able to regenerate scar-free the injured spinal cord. To explore new pathological mechanisms, we compared rat versus axolotl transcriptomics and isolated genes shared between species post-SCI. Unexpectedly, multiple transcripts involved in extracellular matrix remodelling, in particular collagen-1, were upregulated in both species after SCI. Proteomics validated persistent expression of the collagen-enriched matrix signature at the protein level. Collagen-1 accumulated in early and advanced rat lesions. Importantly, collagen-1 was likely associated with pathological vascular remodelling rather than classic fibrosis and the transcription factor SP1 was predicted and validated to regulate, at least in part, the expression of collagen-1 in rat lesions.

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Didangelos, A., Bartus, K., Tica, J., Roschitzki, B., Bradbury, E.. 2017-09-05. Rats and axolotls share a common molecular signature after spinal cord injury enriched in collagen-1. https://doi.org/10.1101/184713

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