An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues

Corces, M. R.; Trevino, A. E.; Hamilton, E. G.; Greenside, P. G.; Sinnott-Armstrong, N. A.; Vesuna, S.; Satpathy, A. T.; Rubin, A. J.; Montine, K. S.; Wu, B.; Kathiria, A.; Cho, S. W.; Mumbach, M. R.; Carter, A. C.; Kasowski, M.; Orloff, L. A.; Risca, V. I.; Kundaje, A.; Khavari, P. A.; Montine, T. J.; Greenleaf, W. J.; Chang, H. Y.

doi:10.1101/181206

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bioRxiv · 10.1101/181206

An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues

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Abstract

We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol enables chromatin accessibility profiling from archival frozen tissue samples and 50 m sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.

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Corces, M. R., Trevino, A. E., Hamilton, E. G., Greenside, P. G., Sinnott-Armstrong, N. A., Vesuna, S., Satpathy, A. T., Rubin, A. J., Montine, K. S., Wu, B., Kathiria, A., Cho, S. W., Mumbach, M. R., Carter, A. C., Kasowski, M., Orloff, L. A., Risca, V. I., Kundaje, A., Khavari, P. A., Montine, T. J., Greenleaf, W. J., Chang, H. Y.. 2017-08-26. An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues. https://doi.org/10.1101/181206

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