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bioRxiv · 10.1101/179622

Low-level mitochondrial heteroplasmy modulates DNA replication, glucose metabolism and lifespan in mice

Abstract

Mutations in mitochondrial DNA (mtDNA) lead to heteroplasmy, i.e. the intracellular coexistence of wild-type and mutant mtDNA strands, which impact a wide spectrum of diseases but also physiological processes, including endurance exercise performance in athletes. However, the phenotypic consequences of limited levels of naturally-arising heteroplasmy have not been experimentally studied to date. We hence generated a conplastic mouse strain carrying the mitochondrial genome of a AKR/J mouse strain (B6-mtAKR) together with a C57BL/6J nuclear genomic background, leading to >20% heteroplasmy in the origin of light-strand DNA replication (OriL). These conplastic mice demonstrate a shorter lifespan as well as dysregulation of multiple metabolic pathways, culminating in impaired glucose metabolism, compared to wild-type C57BL/6J mice carrying lower levels of heteroplasmy. Our results indicate that physiologically relevant differences in mtDNA heteroplasmy levels at a single, functionally important site impair metabolic health and lifespan in mice.\n\nHighlightsO_LIWe identify heteroplasmy of the adenine-repeat variation (9 to 13A) in nt5172 in the origin of light-strand DNA replication (OriL) in inbred mice.\nC_LIO_LIB6-mtAKR mice carry >20% 12A heteroplasmy in the OriL, while B6 mice carry only [~] 10% heteroplasmy.\nC_LIO_LIThe level of 12A heteroplasmy correlates to mtDNA copy number, glucose metabolism, and lifespan in mice.\nC_LIO_LIGiven the established role of mtDNA heteroplasmy in regards to endurance exercise performance in athletes, these findings may impact our understanding of metabolism and aging in humans.\nC_LI

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Hirose, M., Schilf, P., Gupta, Y., Zarse, K., Kuenstner, A., Busch, H., Yin, J., Wright, M. N., Ziegler, A., Vallier, M., Belheouane, M., Baines, J. F., Tautz, D., Johann, K., Oelkrug, R., Mittag, J., Lehnert, H., Othman, A., Joehren, O., Schwaninger, M., Prehn, C., Adamski, J., Shima, K., Rupp, J., Haesler, R., Fuellen, G., Koehling, R., Ristow, M., Ibrahim, S. M.. 2017-08-22. Low-level mitochondrial heteroplasmy modulates DNA replication, glucose metabolism and lifespan in mice. https://doi.org/10.1101/179622

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