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bioRxiv · 10.1101/177568

Single-cell RNA sequencing reveals cell-type specific cis-eQTLs in peripheral blood mononuclear cells

van der Wijst, M. G. P.·Brugge, H.·de Vries, D. H.·Franke, L. H.
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Abstract

Most disease-associated genetic risk factors are regulatory. Here, we generated single-cell RNA-seq data of [~]25,000 peripheral blood mononuclear cells from 45 donors to identify how genetic variants affect gene expression. We validated this approach by replicating previously published whole blood RNA-seq cis-expression quantitative trait loci effects (cis-eQTLs), but also identified new cell type-specific cis-eQTLs. These eQTLs give additional insight into the downstream consequences of genetic risk factors for immune-mediated diseases.

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van der Wijst, M. G. P., Brugge, H., de Vries, D. H., Franke, L. H.. 2017-08-17. Single-cell RNA sequencing reveals cell-type specific cis-eQTLs in peripheral blood mononuclear cells. https://doi.org/10.1101/177568

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