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bioRxiv · 10.1101/177097

PTE, a novel module of mammalian Polycomb Response Elements

Cameron, S. R.·Nandi, S.·Kahn, T. G.·Barrasa, J. I.·Stenberg, P.·Schwartz, Y. B.
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Abstract

Polycomb Group proteins are essential epigenetic repressors. They form multiple protein complexes of which two kinds, PRC1 and PRC2, are indispensable for repression. Although much is known about their biochemical properties, how PRC1 and PRC2 are targeted to specific genes is poorly understood. Here we establish the Cyclin D2 (CCND2) oncogene as a simple model to address this question. We provide the evidence that coordinated recruitment of PRC1 and PRC2 complexes to CCND2 involves a combination of a specialized PRC1 targeting element (PTE) and an adjacent CpG-island, which together act as a human Polycomb Response Element. Chromatin immunoprecipitation analysis of CCND2 in different transcriptional states indicates that histone modifications produced by PRC1 and PRC2 are not sufficient to recruit either of the complexes. However, catalytic activity of PRC2 helps to anchor PRC1 at the PTE. Our analyses suggest that coordinated targeting of PRC1 and PRC2 complexes by juxtaposed AT-rich PTEs and CpG-islands may be a general feature of Polycomb repression in mammals.

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Cameron, S. R., Nandi, S., Kahn, T. G., Barrasa, J. I., Stenberg, P., Schwartz, Y. B.. 2017-08-16. PTE, a novel module of mammalian Polycomb Response Elements. https://doi.org/10.1101/177097

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