First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (formerly Scedosporium prolificans)

Luo, R.; Zimin, A.; Workman, R.; Fan, Y.; Pertea, G.; Grossman, N.; Wear, M.; Jia, B.; Miller, H.; Casadevall, A.; Timp, W.; Zhang, S.; Salzberg, S.

doi:10.1101/171975

bioRxiv · 10.1101/171975

First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (formerly Scedosporium prolificans)

Luo, R.·Zimin, A.·Workman, R.·Fan, Y.·Pertea, G.·Grossman, N.·Wear, M.·Jia, B.·Miller, H.·Casadevall, A.·Timp, W.·Zhang, S.·Salzberg, S.

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Abstract

Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,630,066 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8,656 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.

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Luo, R., Zimin, A., Workman, R., Fan, Y., Pertea, G., Grossman, N., Wear, M., Jia, B., Miller, H., Casadevall, A., Timp, W., Zhang, S., Salzberg, S.. 2017-08-03. First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (formerly Scedosporium prolificans). https://doi.org/10.1101/171975

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