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bioRxiv · 10.1101/155069

Xolik: finding cross-linked peptides with maximum paired scores in linear time

Dai, J.·Jiang, W.·Yu, F.·Yu, W.
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Abstract

MotivationCross-linking technique coupled with mass spectrometry (MS) is widely used in the analysis of protein structures and protein-protein interactions. In order to identify cross-linked peptides from MS data, we need to consider all pairwise combinations of peptides, which is computationally prohibitive when the sequence database is large. To alleviate this problem, some heuristic screening strategies are used to reduce the number of peptide pairs during the identification. However, heuristic screening criteria may ignore true findings.\n\nResultsWe directly tackle the combination challenge without using any screening strategies. With the additive scoring function and the data structure of double-ended queue, the proposed algorithm reduces the quadratic time complexity of exhaustive searching down to the linear time complexity. We implement the algorithm in a tool named Xolik, and the running time of Xolik is validated using databases with different number of proteins. Experiments using synthetic and empirical datasets show that Xolik outperforms existing tools in terms of running time and statistical power.\n\nAvailabilitySource code and binaries of Xolik are freely available at http://bioinformatics.ust.hk/Xolik.html.\n\nContacteeyu@ust.hk\n\nSupplementary informationSupplementary data are available at Bioinformatics online.

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Dai, J., Jiang, W., Yu, F., Yu, W.. 2017-06-24. Xolik: finding cross-linked peptides with maximum paired scores in linear time. https://doi.org/10.1101/155069

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