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bioRxiv · 10.1101/142075

Use Of RetroNectin In Studies Requiring In Vitro HIV Infection Of Human Hematopoietic Stem/Progenitor Cells

Tsukamoto, T.·Okada, S.
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Abstract

Human immunodeficiency virus (HIV) causes damage, directly or indirectly, to the whole hematopoietic system including CD34+ hematopoietic stem/progenitor cells (HSPC). CXCR4-tropic strains of HIV-1 may be potent to affect the function of CD34+CXCR4+ progenitor cells either by infecting the cells or by modifying the dynamics of more differentiated hematopoietic cells. However, CD34+ cells are known for the resistance to HIV-1 infection in vitro, restricting the detailed analysis of the impact of HIV upon HSPC. Here the authors report a use of RetroNectin, a recombinant fibronectin fragment used for gene transfer with lentiviral vectors, to overcome the limitation.

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Tsukamoto, T., Okada, S.. 2017-05-25. Use Of RetroNectin In Studies Requiring In Vitro HIV Infection Of Human Hematopoietic Stem/Progenitor Cells. https://doi.org/10.1101/142075

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