Targeting Repair Pathways With Small Molecules Increases Precise Genome Editing In Pluripotent Stem Cells

Riesenberg, S.; Maricic, T.

doi:10.1101/136374

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bioRxiv · 10.1101/136374

Targeting Repair Pathways With Small Molecules Increases Precise Genome Editing In Pluripotent Stem Cells

Riesenberg, S.·Maricic, T.

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Abstract

Introductory paragraph Introductory paragraph Main text Author contributions Competing Interests Cell culture Generation and validation of... Small molecules Design of gRNAs and... Lipofection of oligonucleotides Ribonucleoprotein nucleofection FACS-sorting Illumina library preparation and... CRISPResso analysis Statistical analysis Online Methods References Online methods references A now frequently used method to edit mammalian genomes uses the nucleases CRISPR/Cas9 and CRISPR/Cpf1 or the nickase CRISPR/Cas9n to introduce double-strand breaks (DSBs) which are then repaired ...

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Riesenberg, S., Maricic, T.. 2017-05-10. Targeting Repair Pathways With Small Molecules Increases Precise Genome Editing In Pluripotent Stem Cells. https://doi.org/10.1101/136374

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