Low-Cost HPV Screening And The Prevalence Of Cervical Infection In Asymptomatic Populations In Guatemala

Lou, H.; Gharzouzi, E.; Guerra, S. P.; Domgue, J. F.; Sawitzke, J.; Villagran, G.; Garland, L.; Boland, J.; Wagner, S.; Martinez, J.; Rosas, H.; Troxler, J.; McMillen, H.; Kessing, B.; Alvirez, E.; Castillo, M.; Morales, H.; Argueta, V.; Rosingh, A.; van Aerde-van Nunen, F.; Lopez, G.; Schiffman, M.; Dean, M.; Orozco, R.

doi:10.1101/134775

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bioRxiv · 10.1101/134775

Low-Cost HPV Screening And The Prevalence Of Cervical Infection In Asymptomatic Populations In Guatemala

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Abstract

BackgroundA low cost and accurate method for detecting high-risk (HR) HPV is important to permit HPV testing for cervical cancer prevention. We validated a low-cost commercially available HPV method (H13, Hybribio, Hong Kong) and determined the distribution of HPV infections in over 1717 cancer-free women in Guatemala.\n\nMethodsH13 results were compared with two more established HPV tests: (Xpert (Cepheid) and SPF10-LIPA25 (DDL)) in 40 mainly known positive specimens. HR-HPV was detected in cervical samples from 1717 cancer-free women receiving Pap smears using the Hybribio realtime PCR assay of 13 HR types. Selected HPV positive samples were sequenced to determine viral type.\n\nResultsThe Hybribio H13 Assay showed 93% identical results with Xpert, and 89% with SPF10-LIPA25. A total of 13% (226/1717) of women tested HPV+. The highest prevalence was found in younger women (<30 years, 22 %) and older ones ([≥]60 years, 15%). The six most common HR-HPV types among the 148 HPV+ typed were HPV16 (22%), HPV18 (11%), HPV39 (11%), HPV58 (10%), HPV52 (8%), and HPV45 (8%).\n\nConclusionsThe Hybribio Assay was low cost, and accurate in testing for HR-HPV infection. As in most places, HPV16 was the most prevalent HR type in Guatemala and the age-specific prevalence curve peaked in younger ages.\n\nHighlightsO_LIA low cost and accurate method, Hybribio Assay, could be used for CC testing in low income regions.\nC_LIO_LIA total of 13% of cancer-free women were HPV+ and positivity was associated with younger age (<30 years old) in Guatemala.\nC_LIO_LIHPV16 was the major prevalent type.\nC_LI

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Lou, H., Gharzouzi, E., Guerra, S. P., Domgue, J. F., Sawitzke, J., Villagran, G., Garland, L., Boland, J., Wagner, S., Martinez, J., Rosas, H., Troxler, J., McMillen, H., Kessing, B., Alvirez, E., Castillo, M., Morales, H., Argueta, V., Rosingh, A., van Aerde-van Nunen, F., Lopez, G., Schiffman, M., Dean, M., Orozco, R.. 2017-05-08. Low-Cost HPV Screening And The Prevalence Of Cervical Infection In Asymptomatic Populations In Guatemala. https://doi.org/10.1101/134775

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