Single Molecule Sequencing Of M13 Virus Genome Without Amplification

Zhao, L.; Deng, L.; Li, G.; Jin, H.; Cai, J.; Shang, H.; Li, Y.; Wu, H.; Xu, W.; Zeng, L.; Zhang, R.; Zhao, H.; Wu, P.; Zhou, Z.; Zheng, J.; Ezanno, P.; Yan, Q.; Deem, M.; He, J.

doi:10.1101/133843

bioRxiv · 10.1101/133843

Single Molecule Sequencing Of M13 Virus Genome Without Amplification

Zhao, L.·Deng, L.·Li, G.·Jin, H.·Cai, J.·Shang, H.·Li, Y.·Wu, H.·Xu, W.·Zeng, L.·Zhang, R.·Zhao, H.·Wu, P.·Zhou, Z.·Zheng, J.·Ezanno, P.·Yan, Q.·Deem, M.·He, J.

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Abstract

Third generation sequencing is a direct measurement of DNA/RNA sequences at the single molecule level without amplification. In this study, we report sequencing of the genome of the M13 virus by a new single molecule sequencing platform. Our platform detects single molecule fluorescence by the total internal reflection microscope technique, with sequencing-by-synthesis chemistry. We sequenced the genome of M13 to a depth of 316x and 100% coverage. The consensus sequence accuracy is 100%. We demonstrated that single molecule sequencing has no significant GC bias.

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Zhao, L., Deng, L., Li, G., Jin, H., Cai, J., Shang, H., Li, Y., Wu, H., Xu, W., Zeng, L., Zhang, R., Zhao, H., Wu, P., Zhou, Z., Zheng, J., Ezanno, P., Yan, Q., Deem, M., He, J.. 2017-05-03. Single Molecule Sequencing Of M13 Virus Genome Without Amplification. https://doi.org/10.1101/133843

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