The WPRE Improves Genetic Engineering With Site-Specific Nucleases

Ong, J. M.; Brown, C. R.; Mendel, M. C.; Cost, G. J.

doi:10.1101/126904

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bioRxiv · 10.1101/126904

The WPRE Improves Genetic Engineering With Site-Specific Nucleases

Ong, J. M.·Brown, C. R.·Mendel, M. C.·Cost, G. J.

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Abstract

AbstractInclusion of the woodchuck hepatitis virus post-transcriptional response element (WPRE) in the 3 UTR of mRNA encoding zinc-finger or TALE nucleases results in up to a fifty-fold increase in nuclease expression and a several-fold increase in nuclease-modified chromosomes. Significantly, this increase is additive with the enhancement generated by transient hypothermic shock. The WPRE-mediated improvement is seen across several types of human and mouse primary and transformed cells and is translatable in vivo to the mouse liver.

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Ong, J. M., Brown, C. R., Mendel, M. C., Cost, G. J.. 2017-04-12. The WPRE Improves Genetic Engineering With Site-Specific Nucleases. https://doi.org/10.1101/126904

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